The aim of this research was to analyze the differences in individual dimensions of anthropological status, motor skills and ball skills tests among pioneer soccer players. 60 football players in the pioneer category from three football clubs participated in the research: NK Croatia Zmijavci, NK Adriatic and NK Orkan. After the measurements, the sample was divided into two groups: biologically more mature (N=27) and biologically immature group (N=33). The sample of variables was made on a sample of 11 variables, which were divided into 4 groups: anthropometric characteristics, motor skills, variables for assessing technique with the ball and index of football skill. Descriptive indicators were calculated for the entire sample, but also for separate groups of biologically mature and immature. A T-test for independent samples was also used to determine the differences between the observed groups. Arithmetic mean, standard deviation, minimum and maximum results were calculated for each measurement. With regard to the set goal of the research and research hypotheses, the results showed that the hypotheses were met. There are statistically significant differences between biologically mature and immature players in the variables for assessing anthropometry and there are statistically significant differences between biologically mature and immature players in the variables for assessing ability with the ball accepted. The hypothesis that there are statistically significant differences between biologically mature and immature players in the variables for assessing motor skills was partially accepted. In all anthropometric measurements, the biologically more mature group has higher results than the biologically immature group, thus a statistically significant difference was obtained in the variables body height, sitting height, body mass and body mass index. A statistically significant difference was also obtained in the measurement of motor skills, i.e. in the long jump from a standing position and the medicine ball throw with a squat jump, where the biologically mature group achieves better results than the biologically immature group. Also, significant differences were obtained when measuring the ability to guide the ball, where a statistically significant difference exists when measuring the sprint 20 m with the ball and the slalom test with the ball. In these measurements, the biologically immature group achieves better results compared to the biologically mature group.