Sports diagnostics is a series of procedures that seek to define as clearly as possible the characteristics of athletes in order to optimize sports training. The aim of this study is to determine whether there is muscle imbalance in football players with respect to the dominant and non-dominant sides. The study used a sample of 47 healthy professional soccer players seniors from HNK Rijeka and football club FK Titograd. The average age of the team is 22.89 ± 5.11; weight 76.58 ± 6.32 kg and height 182.30 ± 5.82 cm. The method we used in the study is tensiomyography (TMG). The variables used in the study are: contraction delay time (𝑇𝑑), contraction time (𝑇𝑐), maximal displacement of the abdominal muscle (𝐷𝑚), and contraction rate (𝑉𝑐). Muscle tested: TA (tibialis anterior), SO (soleus), BF (biceps femoris), RF (rectus femoris), VL (vastus lateralis), VM (vastus medialis), GL (gastrocnemius lateralis), GM (gastrocnemius medialis) , AL (adductor longus), RA (rectus abdominus), ES (erector spinae), GT (gluteus maximus), ST (semitendinosus). The results obtained indicate that there is no muscle imbalance with cosidering to the dominant and non-dominant sides. Despite the common belief that unilateral activities cause bilateral imbalances prevail in football, a detailed structural and anatomical analysis of the football game shows that the football game is equally burdensome on both sides of the locomotive apparatus. From the obtained results we can conclude that bilateral imbalance represents an anomaly in the functionality of the locomotor apparatus and that as such we have to repair it.