This study involved testing 8 students from the Faculty of Kinesiology in Split. The aim was to determine the differences between the initial and final measurements in the triceps brachii and serratus anterior muscles during the execution of a front punch, to examine if there is a statistically significant difference in changes in muscle activation of the triceps brachii and serratus anterior on the dominant and non-dominant side during a lead jab, and whether there is a statistically significant difference in changes in muscle activation between the triceps brachii and serratus anterior during the lead jab. Variables in the sample included RMS, iEMG, and activation time. The results showed no statistically significant differences between the initial and final measurements of the activation of the triceps brachii and serratus anterior muscles, except in the case of the triceps brachii on the non-dominant side, where a statistically significant difference was found in activation time. Additionally, no statistically significant differences were found in the changes in muscle activation between the triceps brachii and serratus anterior muscles, nor were there any statistically significant differences in the changes in muscle activation of the triceps brachii and serratus anterior during the front punch between the dominant and non-dominant side. This could be due to participant fatigue before the lengthy measurement and insufficient intervention stimulus. Future research should aim to reduce the influence of external factors on the results, provide a stronger intervention stimulus, and include a larger sample size.