Water polo is a team sport played in water and is recognized for its rapid, explosive changes in direction and speed of swimming, wrestling and shooting, which are crucial components of the game. For this reason, anaerobic endurance and muscle strength of the arm and shoulder girdle are of great importance in water polo. The newly constructed arm Wingate test was created due to the fact that in practice there is not much research in sports in which the requirements are similar to water polo, i.e. the need for strength and endurance of the muscles of the upper body, and the Wingate test, which measures the strength and endurance of the legs, is the most used test. Also, research that used an arm Wingate test that was performed with the subject sitting. In this way, the subject has the possibility to manipulate compensation by using other muscles as well. In newly constructed arm Wingate test those posibilities are eliminated by placing the subjects in a lying position and attaching them to a bench. Since we achieved muscle isolation in the test, we can say that the newly constructed test is valid. Due to its characteristics, the newly constructed test is also applicable for other sports where strength and endurance of the upper body are necessary for top results, such as throwing disciplines in athletics, handball, swimming, and even wrestling. In further research, it will be necessary to include more athletes from several different sports, as well as to monitor some other parameters with which the level of anaerobic endurance can be diagnosed even more precisely.